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Cloning and Expression
Enzymes
KasI
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KasI
KasI from New England Biolabs
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Citations:
(4)
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New England Biolabs for
KasI
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Product Specs
Item
KasI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0544L
Concentration
1-5 u/µL
Quantity
1250 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-80 C
Sequence
G/GCGCC
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (4)
(1)
Rapid production and free distribution of a synthetic RNA material to support SARS-CoV-2 molecular diagnostic testing
Biologicals
May 3, 2023
Megan H. Cleveland, Erica L. Romsos, Carolyn R. Steffen, Nathan D. Olson, Stephanie L. Servetas, William G. Valiant, Peter M. Vallone
fragments, a double
restriction digest
was performed with
KasI
(
New England Biolabs
, Ipswich, MA, catalog #
R0544L
) and
NruI
(
New England Biolabs
, catalog #
R0192L
) to cut the desired construct from the backbone plasmid.
(2)
Use of a Bacterial Artificial Chromosome to Generate Recombinant SARS-CoV-2 Expressing Robust Levels of Reporter Genes
Microbiology Spectrum
December 21, 2022
Chengjin Ye, Luis Martinez-Sobrido
R0104L
), isopropanol (Fisher Scientific, catalog no. BP26181),
KasI
(
New England Biolabs
, catalog no.
R0544L
), Luria-Bertani (LB) agar (BD, catalog no. 214010), Lipofectamine 2000 reagent (
Thermo Fisher Scientific
,
(3)
ALIBY: ALFA Nanobody-Based Toolkit for Imaging and Biochemistry in Yeast
mSphere
October 26, 2022
Dipayan Akhuli, Anubhav Dhar, Aileen Sara Viji, Bindu Bhojappa, Saravanan Palani
the genomic leu2 locus, piSP522, piSP523, piSP524, and piSP525 were linearized with
KasI
(catalog no.
R0544
;
New England BioLabs
) and transformed into the above-described
strains
as appropriate. To construct
(4)
Chimeric Phi29 DNA polymerase with helix–hairpin–helix motifs shows enhanced salt tolerance and replication performance
Microbial Biotechnology
July 1, 2021
Yaping Gao, Yun He, Liyi Chen, Xing Liu, Igor Ivanov, Xuerui Yang, Hui Tian
(residues 518–964) of Topo V after
PCR
were tethered to the COOH terminus of Phi29 Pol using
KasI
(
R0544S
;
NEB
, USA). The sequence of the linker peptide was GTGSGA. The scheme for generating the chimeric gene
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KasI from New England Biolabs
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